To understand the theory and operational principles of instrumental methods for identification and quantitative analysis of chemical substances by different types of spectroscopy.
To impart fundamental knowledge on gas chromatography and liquid chromatography.
To integrate a fundamental understanding of the underlining principles of physics as they relate to specific instrumentation used for gas analyzers and pollution monitoring instruments.
To impart knowledge on the important measurement in many chemical processes and laboratories handling liquids or solutions.
To understand the working principle, types and applications of NMR and Mass spectroscopy.
UNIT I SPECTROPHOTOMETRY 9
Spectral methods of analysis – Beer-Lambert law – UV-Visible spectroscopy – IR Spectrophotometry – FTIR spectrophotometry – Atomic absorption spectrophotometry – Flame emission and atomic emission photometry – Construction, working principle, sources detectors and applications.
UNIT II CHROMATOGRAPHY 9
General principles – classification – chromatographic behavior of solutes – quantitative determination – Gas chromatography – Liquid chromatography – High-pressure liquid chromatography – Applications.
UNIT III INDUSTRIAL GAS ANALYZERS AND POLLUTION MONITORING
Gas analyzers – Oxygen, NO2 and H2S types, IR analyzers, thermal conductivity detectors, analysis based on ionization of gases.
Air pollution due to carbon monoxide, hydrocarbons, nitrogen oxides, sulphur dioxide estimation – Dust and smoke measurements.
UNIT IV pH METERS AND DISSOLVED COMPONENT ANALYZERS 9
Selective ion electrodes – Principle of pH and conductivity measurements – dissolved oxygen analyzer – Sodium analyzer – Silicon analyzer – Water quality Analyzer.
UNIT V NUCLEAR MAGNETIC RESONANCE AND MASS SPECTROMETRY 9
NMR – Basic principles – Continuous and Pulsed Fourier Transform NMR spectrometer – Mass Spectrometry – Sample system – Ionization methods – Mass analyzers – Types of mass spectrometry.
TOTAL : 45 PERIODS
COURSE OUTCOMES (COs)
1 . Ability to understand the fundamental principles of selective analytical
instruments used in medical diagnosis, quality assurance & control and research studies.
- Ability to assess and suggest a suitable analytical method for a specific
purpose, and evaluate sensitivity, important sources of interferences and errors, and also suggest alternative analytical methods for quality assurance.
- Ability to critically evaluate the strengths and limitations of the various instrumental methods.
- Ability to develop critical thinking for interpreting analytical data.
- Ability to understand the working principle, types and applications of NMR and Mass spectroscopy
- Willard, H.H., Merritt, L.L., Dean, J.A., Settle, F.A., ”Instrumental methods of analysis”, CBS publishing & distribution, 7th Edition, 2012.
Braun, R.D., “Introduction to Instrumental Analysis”, Pharma Book Syndicate, Singapore, 2006.
- Robert E. Sherman., “Analytical Instrumentation”, Instruments Society of America, 1996.
- Khandpur, R.S., “Handbook of Analytical Instruments”, Tata McGraw-Hill publishing Co. Ltd.,2nd Edition 2007.
- Ewing, G.W., “Instrumental Methods of Chemical Analysis”, McGraw-Hill, 5th Edition reprint 1985. (Digitized in 2007).
- Liptak, B.G., “Process Measurement and Analysis”, CRC Press, 5th Edition, 2015.
- NPTEL lecture notes on, “Modern Instrumental methods of Analysis” by Dr.J.R. Mudakavi, IISC, Bangalore.